According to the size of the workload, it is mainly selected from two aspects of speed and capacity. The following is a detailed introduction to the problems that should be paid attention to when purchasing precision centrifuges. If you understand this, you can also purchase the hospital laboratory centrifuges yourself~~
(1) Rotating speed: Centrifuge is divided into low speed centrifuge (<1 0000 rpm/min), high speed centrifuge (1 0000 rpm/min 30,000 rpm/min), ultra high speed centrifuge (>30000 rpm) according to the maximum speed. /min), each centrifuge has a rated maximum speed. The maximum speed refers to the speed under no-load conditions, but the maximum speed varies depending on the type of rotor and the quality of the sample. For example, a centrifuge rated at 16,000 rpm/min indicates that the rotor rotates 16,000 times per minute at no load, and after the sample is added, the speed must be less than 16,000 rpm/min. The rotor speed is different, the maximum speed is different (one rotor can be equipped with multiple rotors), the horizontal rotor can reach 1 5000 rpm/min, but the angle rotor can reach 14000 rpm/min. The specific difference should be consulted in detail. The sales personnel and the relevant technical personnel of the production plant, so the choice of the speed should be cautious, the maximum speed of the selected centrifuge is higher than the target speed. For example, the target speed is 16000 rpm/mIn, and the maximum speed of the selected centrifuge must be higher than 16000 rpm/min.
(2) Temperature: Some samples (such as proteins, cells, etc.) will be destroyed under high temperature conditions. This is to choose a refrigerated centrifuge. The refrigerated centrifuge has a rated temperature range. The heat generated by the centrifuge at high speed is balanced with the refrigeration system of the centrifuge at a certain temperature (the sample for general freeze centrifugation needs to be kept at 3 to 8), and the specific amount can be related to the rotor, such as the rated temperature of a centrifuge. The range is -10 to 60, and the horizontal rotor can be rotated to about 3 when it is rotated. If it is an angular rotor, it may only be around 7. This can also be consulted in detail about the sales staff of Intech Instruments and the relevant technical personnel of the production plant.
(3) Capacity: How many sample tubes need to be centrifuged each time 7 How much capacity is needed for each sample tube 7 These factors determine the total capacity of a centrifuge, simply the total volume of the centrifuge = the capacity of each centrifuge tube × centrifugation The number of tubes, the total capacity and the size of the workload are matched.
(4) Rotor: The rotor of the centrifuge is mainly divided into two types. The horizontal rotor: the hanging blue is in a horizontal state during operation, and is at right angles to the rotating shaft. The sample concentrates the sediment on the bottom of the centrifuge tube: the angle rotor: the centrifugal container is fixed with the rotating shaft At the angle, the sample concentrates the sediment on the bottom of the centrifuge tube and the side wall near the bottom. If you want the separated sample to be concentrated at the bottom of the centrifuge tube, select the horizontal rotor. If you want the sample to concentrate on the bottom of the centrifuge tube and the side wall near the bottom, select the angle rotor.
There are also special tests or special samples that require special rotors such as large-capacity baskets (used for blood stations), enzyme-labeled rotors, slide rotors, PCR rotors, test-tube rotors, and capillary rotors. The rotor has a fixed size, which is combined with the capacity of the centrifuge, such as the 36 × 5 ml angle rotor, which determines the type of rotor and determines the capacity of the centrifuge, so the choice of the rotor is very important.
(5) Control system: High-end centrifuges use microcomputer control systems. These control systems not only ensure the safe operation of the centrifuge but also automatically complete the task. Now many centrifuges have a better humanized control system. For example: rotor recognition function, safety lock function, fault prompt function, acceleration and deceleration curves, etc. In addition to the above points, pay attention to some details and necessary accessories. The main components of the centrifuge are motors. The motors are divided into carbon brush motors and carbonless brush motors. The former has been eliminated. Most of the current centrifuges are brushless motors, and some motors also have brake functions. Refrigerated centrifuges also differ in refrigeration, and now environmentally friendly technologies are of course fluorine-free refrigeration. In addition to this, consider the noise problem and try to choose a quieter centrifuge to maintain a comfortable experimental environment. Care should be taken in the accessories. In some experiments, special centrifuge tubes (centrifugal toxic samples or samples requiring ultra-high-speed centrifugation) should be used. Such centrifuge tubes must be equipped with corresponding sleeves for safety. There are also some special sample containers (irregular vials, blood bags, etc.). These details and accessories should be carefully considered when selecting a centrifuge, otherwise normal operation will not be possible.